Primer

Part:BBa_K4926005

Designed by: Wang Leyi   Group: iGEM23_PINGHE   (2023-10-07)


femAsg2

cas12a needs to bind to crRNA to be active, and the sequence of the crRNA complementary to its target DNA determines the recognition targeting of cas12a. This is the primer used for in vitro transcription to get the corresponding crRNA of the target sequence.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None